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. 2009 May 5;7(5):e1000103. doi: 10.1371/journal.pbio.1000103

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© 2009 Shinozaki et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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(A) Overexpression of P2X₄R in human 1321N1 astrocytoma cells. In Western blotting, P2X₄R protein was only detected in cells transfected with the P2X₄R gene.

(B) Purification of P2X₄R protein. The molecular weight of the purified P2X₄R protein band was detected at about 150 kDa in native-PAGE (left) and 50 kDa in SDS-PAGE (right). After electrophoresis, the gels were stained with silver staining.