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. 2009 Feb 12;23(5):689–699. doi: 10.1210/me.2008-0350

Figure 4.

Figure 4

Adrenocorticoids affect the expression of TRPCs in isolated chromaffin cells from the adrenal medulla of metabolic syndrome pigs. A, TRPC RNAs are quickly degraded in isolated chromaffin cells. RT-PCR analysis of TRPC expression in isolated chromaffin cells treated with actinomycin D (AMD) for 24 h vs. untreated cells. B and C, Relative expression of TRPCs in dissociated adrenal chromaffin cells. RT-PCR analysis was performed using total RNA isolated from chromaffin cells treated with indicated chemicals for 24 h in a serum-free medium. A, Aldosterone; C, control; DM, dexamethasone; SL, spironolactone. β-Actin normalized data are plotted. Representative images of ethidium bromide-containing agarose gels are shown in B. *, Significant difference between tested groups (P < 0.05; n = 4–9).