Figure 1.
Association of ASCOM and Swi/Snf in the nuclear matrix. A, ASC-2 and BRG1 are highly colocalized in the nucleus. B, Like the nuclear matrix protein lamin B, ASC-2, ASH2, and BRG1 were found associated with the nuclear matrix. Similar results were obtained for RBQ-3, another component of ASCOM (data not shown). C, Fractionations of nuclear extracts into three parts revealed that lamin B, BRG1, ASC-2, ASH2, and RBQ-3 are enriched in the nuclear matrix. D, Schematic representation of wild-type INI1 and ΔINI1 is as shown. GST alone or GST fusion to INI1 or MLL3–4591-4904 were tested for binding 35S-labeled, in vitro-translated MLL4-4744-5262, INI, or ΔINI1, as indicated.