Table 1.

Alignment of substrate-binding envelopes of selected prokaryotic and eukaryotic NATs

TMD no.	11111-333-666666-888	Phenotype
AgNAT6	LS-LG-V-Y-FF-T-F-S-G	Trp specific
AgNAT8	MS-LG-L-Y-FF-S-F-S-A	Phe specific
AeAAT1	MS-LG-V-Y-FS-S-M-S-A	Phe broad
TnaT	AA-LG-V-Y-FF-T-V-S-S	Trp specific
LeuT	NA-LG-V-Y-FF-S-F-S-A	Leu broad
Sites:	dc-cc-c-c-cc-i-c-c-u

NAT, nutrient amino acid transporter; Ae, Aedes aegypti; Ag, Anopheles gambiae; TMD, transmembrane domain

Substrate-interacting sites: c, conserved in AgNAT6 and AgNAT8; d, different in AgNAT6 and AgNAT8; i, identical in Trp-specific eukaryotic and prokaryotic transporters; u, unique in AgNAT6