Figure 4.

(A) The figure graphically summarizes the evolutionary flow of chromosomal changes of human chromosome 6 in primates. ENCs (Evolutionary New Centromere) are indicated by the N in a red circle. The letters on the left of each chromosome indicate the BACs used in the study that are reported in Table 1. The acronyms indicate the primate species as reported in the Methods. The rearrangements that intervened between the Hominoidea ancestral form to HLA20 are not illustrated (for details, see Misceo et al. 2008). The Lar gibbon chromosome bearing the remaining portion of chromosome 6 (HLA3) is not reported because it is not relevant for the position of the centromere. (B) Examples of FISH experiments, in human and in marmoset, of the two BAC clones RP11-297M4 (red) and RP11-1021F13 (green), showing that the clone RP11-1021F13 maps on the tip of the CJA4 short arm because of an inversion (for details, see text). (PA) Primate Ancestor; (STREP) Strepsirrhini; (HLA) Hylobates lar; (HOM) Hominoidea; (H-P-G) Homo-Pan-Gorilla group; (AC) Ancestral Centromere.