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. 2009 May;19(5):913–921. doi: 10.1101/gr.086876.108

Figure 5.

Figure 5.

Characterization of the EI mRNA isoform of TFIIIA. (A) In vitro translation of Arabidopsis EI mRNA. Lanes (1–6) 0, 0.5, 1, 2, 4, and 8 μg EI mRNA, respectively, added in a cell-free wheat germ extract. (B) In vivo translation of EI. Western blot using anti-HA antibody (top) and SDS-PAGE analysis (bottom) of the overexpression lines using L5-cDNA (left) and EI-cDNA (right) fused with HA-tag. Electrophoretic mobility shift assay using GST-LeTFIIIA(zf1-9) (C) and GST-LeTFIIIA(zf1-2) (D). 5S DNA was titrated with increasing concentrations of GST-LeTFIIIA(zf1-9) (lanes 1–9: 0, 0.2, 0.4, 0.9, 1.8, 3.5, 7, 14, and 28 nM, respectively) or GST-LeTFIIIA(zf1-2) (lanes 1–8: 0, 14, 28, 55, 110, 220, 440, and 880 nM, respectively).