FIGURE 10.
Metal inducers of Mt1 bind to MTF1 C-terminal cysteine residues. A, binding with As3+ in vitro. TnT HA-MTF1321–675 was incubated with As3+ (10 μm) for 2 h and then pulled down with PAO and blotted with anti-HA. B, binding with As3+ in vivo. MTF1 KO cells were transfected with pMTF1321–675 HA.His and treated with As3+ at 10 μm for 2 h. MTF1321–675 was pulled down with PAO and blotted with anti-HA. C, binding with other metal inducers in vivo. MTF KO cells transfected with pMTF1321–675 HA.His were treated with Zn2+ (150 μm), Cd2+ (10 μm), Co2+ (150 μm), Ni2+ (150 μm), Ag+ (10 μm), Hg2+ (10 μm), or Bi3+ (50 μm) for 2 h. MTF1321–675 was pulled down with PAO and blotted with anti-HA.