Induction of Mt1 by arsenic requires MTF1. A, Nrf2
WT and KO cells were treated with tBHQ (30 μm), or
As3+ (1, 5 and 10 μm) for 5 h. Total RNA was blotted
with Mt1, Nqo1, and actin riboprobes. B,
MTF1 WT, MTF1 KO, and MTF1 KO cells stably transfected with
pMTF11–675 were treated with As3+ (10
μm) for 5 h. Total RNA was blotted for Mt1,
Ho1, and actin mRNA expression.