FIGURE 3.
Induction of MRE-β-gal reporter by arsenic. BHK3038 were treated with metals for 5 h. Total cell lysate was measured forβ-gal activity as described under “Experimental Procedures.” A–C, concentration curves of β-gal induction by As3+, Cd2+, or Zn2+, respectively. D, BHK3038 cells were transfected with pcDNA3.1HA.his (vector control) or pMTF11–320 (dominant negative MTF1) for 48 h and were treated with As3+ (1, 2, 5, and 10 μm), Cd2+ (10 μm), tBHQ (30 μm), or Zn2+ (100 μm) for 5 h. β-gal activity was measured.