FIGURE 4.
Recruiting MTF1 to endogenous MRE by As3+. A, immunoblotting of MTF1. Cell lysate from hepa1c1c7, in vitro transcription/translation-produced MTF1 (TnT MTF1), and cell lysate from HEK293T cells transfected with pMTF11–675 were blotted with anti-MTF1 antibodies. B, ChIP assay. Hepa1c1c7 cells were treated with tBHQ (30 μm) or As3+ (10 μm) for 5 h. ChIP was performed and precipitated DNA was amplified by real time PCR with MRE primers. Quantitative data represent means and standard deviations from three experiments. Normal rabbit IgG was used as a negative control.