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Superinduction of Mt1 by As3+ and CHX. A, Hepa1c1c7 or BHK3038 cells were treated with CHX (10 μg/ml), arsenic (10 μm), or both for 5 h. Total RNA was blotted for mRNA expression of Mt1 or β-Gal. Gapdh and actin were measured as loading controls. B, MTF1 WT or KO cells were treated with arsenic (10 μm), CHX (10 μg/ml), or both for 5 h. Expression of Mt1 and actin mRNAs was measured by Northern blotting.
