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. 2009 May 8;284(19):12609–12621. doi: 10.1074/jbc.M901204200

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FIGURE 9. — PAO directly binds to the C-terminal cysteine cluster of MTF1. A, biotin-labeled MTF1_1–675 and MTF1_1–320 was produced in vitro in TnT reticulocyte lysate and detected with streptavidin (upper panel). The proteins were pulled down with PAO matrix and detected with streptavidin (lower panel). B, MTF1_321–675 and MTF1_321–631 with a HA tag were produced in vitro and detected with an anti-HA antibody (upper panel). The proteins were pulled down with PAO matrix and detected with anti-HA (lower panel). C, HA-MTF1_321–675 and HA-MTF1_321–675 with all five cysteine residues mutated to phenylalanine were produced in vitro. The proteins were pulled down with PAO and detected with anti-HA. “5CysMut” = C632FC634FC636FC638FC653F.