FIGURE 5.

Inhibition of antigen-specific T cell activation with 2D7 and M17/4. CD4⁺ T cells from OT-II mice (A) or CD8⁺ T cells from OT-I mice (B) were treated with Ova (1, 5, and 20 μg/ml) or SIINFEKL (0.1, 0.01, and 0.001 μg/ml) peptide, respectively, in the presence of APCs. Antibody 2D7 or M17/4 or isotype control at a concentration of 1 μg/ml was added for 4 days. T cells were restimulated with plate-bound anti-CD3. IL-2 was measured 24 h after treatment. Proliferation was assayed at 72 h for OT-II cells and 48 h for OT-I cells after treatment by adding [³H]thymidine to the culture for the last 8 h.