FIGURE 2.

ILY disengagement from hCD59 occurs after establishment of the prepore complex and coincides with pore formation. Antibody accessibility was used to determine whether ILY disengaged from hCD59 during the cytolytic mechanism. Three monoclonal anti-hCD59 antibodies, 10G10 (A), H19 (B), and MEM43 (C), were tested for the ability to bind hCD59 on erythrocytes preincubated with saturating levels of ILY^ml, ILY^pp, or ILY^wt (shaded peaks). As a positive control, each antibody was tested for the ability to bind hCD59 in the absence of toxin (bold line). Background fluorescence of erythrocytes is shown (dashed line). This figure is representative of triplicate experiments.