TABLE 1.
Identification of the 22 kDa band generated by limited tryptic digestion of GAPDH
The band was identified by mass spectrometry with an error of 20 ppm, a score of 103, and 31% of coverage of the full-length of the protein. The asterisks correspond to oxidized methionine. The periods correspond to the residue after which trypsin cleaves the sequence.
| Fragments | Observed Mr | Theoretical Mr | Δ | Miscleavage | Sequence |
|---|---|---|---|---|---|
| ppm | |||||
| 4-13 | 1101.6333 | 1101.6407 | -7 | 1 | K.IRVAINGFGR.I |
| 6-13 | 832.4259 | 832.4555 | -36 | 0 | R.VAINGFGR.I |
| 50-62 | 1386.6770 | 1386.6667 | 7 | 0 | K.YDSTLGTFAADVK.I |
| 50-74 | 2652.2639 | 2652.2919 | -11 | 1 | K.YDSTLGTFAADVKIVDDSHISVDGK.Q |
| 63-74 | 1283.6387 | 1283.6358 | 2 | 0 | K.IVDDSHISVDGK.Q |
| 78-90 | 1537.8308 | 1537.8616 | -20 | 1 | K.IVSSRDPLQLPWK.E |
| 83-90 | 995.5795 | 995.5440 | 36 | 0 | R.DPLQLPWK.E |
| 108-120 | 1222.6667 | 1222.6782 | -9 | 1 | K.VGAGKHIQAGASK.V |
| 129-145 | 1894.9389 | 1894.9313 | 4 | 1 | K.DKDIPTFVVGVNEGDYK.H |
| 172-190 | 2115.0255 | 2115.0055 | 9 | 1 | K.FGIVKGTM*TTTHSYTGDQR.L |
| 177-190 | 1554.7012 | 1554.6733 | 18 | 0 | K.GTMTTTHSYTGDQR.L |
| 177-190 | 1570.7070 | 1570.6682 | 25 | 0 | K.GTM*TTTHSYTGDQR.L |
| 191-197 | 810.4040 | 810.4348 | -38 | 0 | R.LLDASHR.D |
| 191-200 | 1194.6477 | 1194.6469 | 1 | 1 | R.LLDASHRDLR.R |