TABLE 2.
Identification of the 13 kDa band generated by limited tryptic digestion of GAPDH
The band was identified by mass spectrometry with an error of 14 ppm, a score of 115, and 32% coverage of the full length of the protein. The asterisks correspond to oxidized methionine.
| Fragments | Observed Mr | Theoretical Mr | Δ | Miscleavage | Sequence |
|---|---|---|---|---|---|
| ppm | |||||
| 204-218 | 1397.7907 | 1397.7878 | 2 | 0 | R.AAALNIVPTTTGAAK.A |
| 219-228 | 1025.6501 | 1025.6485 | 2 | 0 | K.AVSLVLPSLK.G |
| 229-237 | 940.5654 | 940.5818 | -17 | 1 | K.GKLNGIALR.V |
| 231-237 | 755.4557 | 755.4653 | -13 | 0 | K.LNGIALR.V |
| 231-254 | 2545.4693 | 2545.4843 | -6 | 1 | K.LNGIALRVPTPTVSVVDLVVQVEK.K |
| 238-254 | 1808.0009 | 1808.0296 | -16 | 0 | R.VPTPTVSVVDLVVQVEK.K |
| 238-255 | 1936.1715 | 1936.1245 | 24 | 1 | R.VPTPTVSVVDLVVQVEKK.T |
| 255-266 | 1381.6858 | 1381.6990 | -10 | 1 | K.KTFAEEVNAAFR.E |
| 256-266 | 1253.5926 | 1253.6040 | -9 | 0 | K.TFAEEVNAAFR.E |
| 267-274 | 832.4085 | 832.3749 | 40 | 0 | R.EAANGPM*K.G |
| 267-290 | 2552.2904 | 2552.2945 | -2 | 1 | R.EAANGPM*KGVLHVEDAPLVSIDFK.C |
| 275-290 | 1737.9454 | 1737.9301 | 9 | 0 | K.GVLHVEDAPLVSIDFK.C |
| 313-326 | 1771.7954 | 1771.7954 | 0 | 0 | K.VVAWYDNEWGYSQR.V |
| 327-336 | 1043.5812 | 1043.5863 | -5 | 0 | R.VVDLAEVTAK.K |
| 327-337 | 1171.6715 | 1171.6812 | -8 | 1 | R.VVDLAEVTAKK.W |