TABLE 1.
Substrate specificity of PapA3
ND means not detectable.
| Substrate | Product formationa |
|---|---|
| Nucleophileb | |
| Trehalose | Yes |
| T2P | Yes |
| T3P | ND |
| Trehalose 2-sulfate | ND |
| α,β-Trehalose | ND |
| Glucose | ND |
| Maltose | ND |
| Acyl-CoAc | |
| Palmitoyl-CoA | Yes |
| Docosanoyl-CoA | Yes |
| Butyryl-CoA | ND |
| Crotonoyl-CoA | ND |
a
Product formation was assessed by TLC and phosphorimaging.
b
Reactions were performed with 2 μm enzyme, 20 μm 14C-palmitoyl-CoA, and 1 or 10 mm of each substrate in 100 mm ammonium bicarbonate, pH 7.2, at room temperature for 2 h.
c
Reactions were performed with 2 μm enzyme, 20 μm 14C-acyl-CoA, and 1 mm of trehalose or T2P in 100 mm ammonium bicarbonate, pH 7.2, at room temperature for 2 h.