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A single-stranded telomeric tail is required for ternary complex formation. Around 2 ng of 32P-labeled DNA substrates that do not have a single-stranded tail (A) or have a single-stranded tail with random sequences (B) were incubated with Yku or Cdc13(451–693)p and analyzed by EMSA. The amounts of Yku and Cdc13(451–693)p used were the same as those for Fig. 2B. Autoradiograms are shown here.
