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Binding affinity of Yku to DNA substrates
The apparent binding constant for each substrate was determined from an average of three experiments.
| DNA substrate | Description | Length (duplex + single strand tail) | Kd,app |
|---|---|---|---|
| nm | |||
| R5R6 | Duplex | 18 + 0 | 53 ± 13 |
| T11T12 | Duplex | 18 + 0 | 88 ± 28 |
| R7R8 | Tailed-duplex | 18 + 15 | 77 ± 30 |
| T13T14 | Tailed-duplex | 18 + 15 | 186 ± 43 |
