FIGURE 5.
Experimental x-ray scattering from the NNGH-inhibited, FL-MMP-1 and scattering calculated from the following models: curve 1, experimental data with error bars; curves 2 and 3, computed scattering from the crystallographic models of FL-MMP-1 and FL-MMP-12, respectively; and curve 4, a typical fit by the selected ensemble of structures. The logarithm of the scattering intensity is plotted against the momentum transfer. Inset, the frequency of the models with the given Rg in the initial pool of structures with randomized interdomain linkers (FL-MMP-1 (blue) and FL-MMP-12 (magenta)) and in the selected ensembles (FL-MMP-1 (red) and FL-MMP-12 (green); the latter distribution is obtained by the averaging of several EOM runs. All histograms are normalized to the integral value of unity.