FIGURE 4.

Immunohistochemical localization of BH and filaggrin. Immunofluorescence staining for BH (red) and filaggrin (green) in paraffin-embedded sections of human skin tissue was performed. A, nuclei were counterstained with 4′,6-diamidino-2-phenylindole (DAPI). B and C, paraffin-embedded sections of human skin tissue labeled with dual immunofluorescence were subjected to confocal microscopy. BH and filaggrin co-localized in the granular layer. The upper dotted lines and bottom dotted lines indicate the outer borders of the stratum corneum and the border between the epidermis and dermis. D, merged image of all panels A–C. Magnification of ×40.