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Deimination of filaggrin by mPAD3. The human filaggrin repeat unit was deiminated using a mPAD3 (0.17 μm):filaggrin (8.39 μm) molar ratio of 1:50. After incubation for 0, 10, 20, 30, 60, 180, or 360 min, an aliquot of the reaction mixture was incubated at 37 °C for 1 h without (A) or with (B) trypsin, and subjected to SDS-PAGE. Arrows indicate the unmodified filaggrin, arrowheads indicate the deiminated filaggrin, and the asterisk indicates the faint band with a low molecular mass.
