TABLE 3.

Sequences of primers used in the amplification of G. stearothermophilus UvrA and UvrB interaction domains and the construction of mutants The recognition sequences of the restriction enzymes used for cloning of the PCR products are underlined (NdeI and HindIII). The positions of Arg → Glu, Asp → Arg, and Glu → Arg mutations are in bold type. fwd, forward, rev, reverse.

Primers	Sequences (5′ → 3′)
UvrA 131–245 fwd	CGCGGCAGCCATATGCCCATTTGCCCGACGCAC
UvrA 131–245 rev	GGCCGCAAGCTTTTACGAAAAGCCGCAGTACGGAC
UvrB 149–250 fwd	CGCGGCAGCCATATGGGGTCGCCGGAAGAATATCGG
UvrB 149–250 rev	GGCCGCAAGCTTTTACACGAAGTGCGACGCCGG
UvrA-R176E fwd	ATTCGCAAACAAGGGTATGTGGAAGTCCGTATTGACCGCGAGATG
UvrA-R176E rev	CATCTCGCGGTCAATACGGACTTCCACATACCCTTGTTTGCGAAT
UvrA-E185R fwd	CGTATTGACCGCGAGATGCGCCGTTTGACGGGGGACATTGAGCTT
UvrA-E185R rev	AAGCTCAATGTCCCCCGTCAAACGGCGCATCTCGCGGTCAATACG
UvrA-D219R fwd	GGCATCGCCGCCAGGCTTGCCCGTTCGCTTGAGACGGCGCTGAAG
UvrA-D219R rev	CTTCAGCGCCGTCTCAAGCGAACGGGCAAGCCTGGCGGCGATGCC
UvrA-R206E fwd	CATTCGATTGATGTCGTCGTCGACGAAATCATCATCAAAGACGGCATCGCC
UvrA-R206E rev	GGCGATGCCGTCTTTGATGATGATTTCGTCGACGACGACATCAATCGAATG
UvrB-R183E fwd	CTCGTTGACATCCAATACGACGAAAATGACATCGATTTTCGCCGCGG
UvrB-R183E rev	CCGCGGCGAAAATCGATGTCATTTTCGTCGTATTGGATGTCAACGAG
UvrB-D198R fwd	GGCACGTTCCGCGTCCGCGGCCGTGTTGTCGAAATTTTCCCCGCG
UvrB-D198R rev	CGCGGGGAAAATTTCGACAACACGGCCGCGGACGCGGAACGTGCC
UvrB-E215R fwd	GTCGCGCGATGAACATTGCATTCGCGTCCGCTTTTTCGGCGATGAAATCGAG
UvrB-E215R rev	CTCGATTTCATCGCCGAAAAAGCGGACGCGAATGCAATGTTCATCGCGCGAC
UvrB-E222R fwd	GAATTTTTCGGCGATGAAATCCGTCGCATCCGCGAGGTGGACGCC
UvrB-E222R rev	GGCGTCCACCTCGCGGATGCGACGGATTTCATCGCCGAAAAATTC
UvrB-R223E fwd	TTTTTCGGCGATGAAATCGAGGAAATCCGCGAGGTGGACGCCTTA
UvrB-R223E rev	TAAGGCGTCCACCTCGCGGATTTCCTCGATTTCATCGCCGAAAAA