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. 2009 May 8;284(19):12845–12852. doi: 10.1074/jbc.M808759200

FIGURE 1.

FIGURE 1.

Full-length Tau-YFP binds microtubules while MTBR-YFP aggregates in C17.2 neural cells. A, Tau constructs. YFP fusion proteins were created for expression in mammalian cells as follows: full-length Tau (441 amino acids), Tau-YFP, the microtubule-binding region, MTBR-YFP. For bacterial expression, we used either MTBR alone or with a C-terminal hemagglutinin tag, MTBR-HA. B, Tau-YFP co-localizes with tubulin when transfected into C17.2 cells. C, MTBR-YFP does not co-localize with tubulin and spontaneously aggregates when transfected into C17.2 cells. D, Western blot showing similar expression levels of Tau-YFP and MTBR-YFP. Blots were probed with GFP and actin antibodies. E, based on counting transfected cells, 6% of cells have spontaneous aggregation of Tau-YFP, whereas 79% of cells have spontaneous aggregation of MTBR-YFP (n = 4, 100 transfected cells counted per experiment). F, 83% of cells have Tau-YFP that co-localizes with tubulin, whereas 3.5% of cells have MTBR-YFP that co-localizes with tubulin (n = 4, 100 transfected cells counted per experiment). Scale bars, 10 μm.