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. 2009 May 8;284(19):12845–12852. doi: 10.1074/jbc.M808759200

FIGURE 7.

FIGURE 7.

Tau-YFP aggregates transfer between co-cultured cells. A, cells were transfected separately with mCherry or Tau-YFP. The two cell populations were either mixed immediately prior to analysis or co-cultured for 48 h. Co-cultured cells were treated with buffer, monomer, or aggregates, followed by flow cytometry (10,000 cells sorted per condition). tx, treatment. B, quantification of flow cytometry revealed that 0.15% of cells score positive for mCherry and YFP (upper right quadrant of cell plot) when cells were mixed immediately prior to counting. 0.3 and 0.25% of cells are dual positive when cells are treated with buffer (B) or monomer (M), versus 1% when treated with Tau aggregates (A), indicating transfer of aggregated full-length Tau-YFP between cells. *, p < 10-7 (unpaired Student's t test, n = 4, 10,000 cells counted per experiment). C, dual positive cells were collected, fixed, and mounted. Direct visualization indicates a Tau-YFP inclusion (white arrowhead) within an mCherry-expressing cell. Scale bar, 10 μm.