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A known CD45 inhibitor protects B. anthracis-infected macrophages. J774A.1 macrophages were preincubated with DMSO control (1%) or compounds (5 or 10 μm). After 1 h cells were infected with B. anthracis Sterne spores (5 m.o.i.) for 4 h. The cells were stained with membrane impermeant sytox green dye and analyzed by flow cytometry. The percentage of living and dead cells is indicated. Data from a representative experiment, which was repeated three times with similar results, are shown.
