FIGURE 6.

Activation of Nrf2 and Noxa up-regulation by ATO are not mediated by ATO-induced ROS. A, KMS11 was incubated with 100 μm BHA for 16 h. Control and BHA-treated cells were then treated with 1 μm ATO for 6 h. DHE was used for ROS determination. The shaded histogram represents untreated cells while the bold-lined open histograms represent the indicated treatment. B, KMS11 was treated as in A and protein lysates were subjected to Western blot analysis of HO-1, NQO1, Nrf2, Keap1, Noxa, and actin. C, KMS11 was treated as in A, and cell viability was measured by Annexin-V-FITC/PI staining after 48 h of ATO treatment. Percent (%) of control viability was calculated, and data are presented as mean ± S.D. of four independent experiments.