FIGURE 7.

Nrf2 signaling is not protective during ATO-induced apoptosis. A, MM.1s and KMS11 were electroporated with si(-) (negative control), siNrf2, and siKeap1. After 16 h, cells were treated with 2 μm ATO. Silencing of Nrf2 and Keap1 proteins was determined by Western blot at 6 and 24 h after ATO treatment. B, viability was evaluated by Annexin-V-FITC/PI staining. Percent (%) of control (untreated, transfected, UT) viability was plotted versus time (h). Student's t test was used to compare differences between samples, si(-) and experimental samples unless otherwise indicated, with confidence intervals of 95%. ND, no difference; ^*, p < 0.05.