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Immunoblots of wild-type and mutant Spc29 proteins. A, levels of the indicated Spc29 protein when under the control of the endogenous SPC29 promoter detected with a rabbit anti-Spc29 polyclonal antibody; B, overexpressed GFP-Spc29 or GFP-Spc29-T240A in a wild-type strain background detected with an anti-GFP monoclonal antibody. G-6-PDH, anti-glucose-6-phosphate dehydrogenase (loading control).
