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The mRNAs for GABABR subunits are concentrated in the neuronal soma. Rat hippocampal neurons grown in culture were fixed at 14 DIV and processed for FISH with digoxigenin-labeled antisense probes to microtubule-associated protein 2 or the GABABR subunits. The probes were detected using digoxigenin and secondary antibodies conjugated to Cy3. The outline of each neuron is drawn in white. Lower panels correspond to high magnification images of boxed areas above.
