FIGURE 2.

GABA_BR1 and GABA_BR2 are mobile in dendrites. A, FRAP in 14–16 DIV hippocampal neurons expressing GABA_BR1-EGFP or GABA_BR2-EGFP. ROIs were placed over experimental dendrites (arrowheads) and photobleached, and 40 consecutive frames were acquired over a period of 9 min. Selected frames are shown. Scale bars, 10 μm. B, values of photobleached/recovered regions were normalized to control images and plotted against time. GABA_BR1-EGFP (filled circles) and GABA_BR2-EGFP (empty circles). The data is the average of five to six independent measurements ± S. E. (^*, p < 0.05).