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. 2009 May 8;284(19):13077–13085. doi: 10.1074/jbc.M900575200

FIGURE 5.

FIGURE 5.

BiFC reveals abundant heteromeric GABABRs at the plasma membrane. A, 9–14 DIV rat hippocampal neurons grown in culture were cotransfected with Myc-GABABR1-YFP-N and HA-GABABR2-YFP-C. Neurons were fixed and labeled with antibodies to the N-terminal Myc epitope in GABABR1 to detect surface GABABRs (red). GABABR-BiFC was detected without staining (green). Merged images are shown on the right. B, same as above for neurons labeled with anti-KDEL antibodies to detect the ER. C, same as above for neurons labeled with anti-GM130 antibodies to detect the Golgi apparatus. Cell bodies are shown on top and dendrites below. Scale bars A–C: somas, 10 μm; dendrites, 5 μm.