FIGURE 2.

lin9 is essential for zebrafish development. A, schematic representation of the unspliced lin9 transcript. Morpholino targeting sites (lines) and binding sites for PCR primer (arrows) are indicated. B, injection of MO-E1 (7 ng) prevents splicing of intron 1 or results in a cryptic splice variant. Semiquantitative RT-PCR was conducted 72 hpf. C, lin9 morphants display small head and eyes, cardiac edema, and trunk curvature. A 5-base mismatch morpholino (MO-E1mis) did not evoke developmental defects. Images were taken 72 hpf.