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Cell death and G2/M delay in lin9 morphants. Embryos were injected with 7 ng of indicated morpholinos. At 30 and 48 hpf, clutches of 10 embryos were homogenized. For DNA content analysis, cells were stained with propidium iodide and analyzed by flow cytometry. MO-ATG and MO-E1 evoke an increase of cell number in G2/M 30 hpf compared with uninjected or control (MO-E1mis)-injected embryos. The amount of dead cells (<2n) increases from 30 to 48 hpf in the morphants.
