FIGURE 7.

Microarray analysis reveals that mitotic genes are down-regulated in lin9 morphants. A, functional clustering of genes that are down-regulated ≥1.6-fold in E1 relative to E1mis morphants 24 hpf. B, subclassification of LIN9 targets according to their mitotic function. C, validation of newly identified LIN9 targets by RT-qPCR. As controls, RNA from uninjected and ATG morphants were included. D, whole mount in situ hybridization (24 hpf) for pif1, aspm, top2a, and oip5 on MO-E1- and MO-E1mis-injected embryos.