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. 1999 Mar 2;96(5):2204–2209. doi: 10.1073/pnas.96.5.2204

Figure 1.

Figure 1

Induction of the SOS response by human cDNAs is RecA dependent. Presented are two of the cDNAs, DNA ligase IV and the HSP90-terminal segment homolog HSP90C-Ter, that elicited a recA-dependent SOS response. RecA and recA strains harboring each of the indicated cDNAs were first patched to YT + Amp plates and grown overnight at 37°C. These plates were subsequently replica plated to YT + Amp, YT + Amp + X-Gal + TPEG and YT + Amp + X-Gal + TPEG + IPTG and incubated overnight at 37°C. The SOS response gives rise to blue colonies because of the induction of the dinD1∷LacZ reporter construct. The figure shows the YT + Amp + X-Gal + TPEG + IPTG plates only.