Figure 6.
Adoptive transfer of MLN DC form older mice fail to prime a CTL response in vivo. DC from MLN and spleen from 8 week and 9 month old mice were isolated, plated (5×104 cells per well) and pulsed with irradiated E. cuniculi spores (2.5×105 per well). After overnight incubation, cultures were harvested and cells were adoptively transferred to 9 month old mice (5×105 per mouse) via iv route. Fourteen days later, recipient mice were sacrificed, MLN (A) and splenic (B) lymphocytes prepared and assayed for cytotoxicity. Experiment was performed twice with similar results and data are representative of one experiment.