Fig. 5.
(a) The DNA binding activity of nuclear NF-κB p65 in human monocytes following Pam3CSK4 or LPS exposure with or without candesartan was assessed by ELISA method as detailed in Section 2. Values are normalized to mg nuclear protein and expressed as mean ± S.D. *P < 0.05 vs. control; **P < 0.05 vs. LPS/PAM3CSK4; n = 4 experiments in duplicate. (b) Electrophoretic mobility gel shift assay of nuclear NF-κB in human monocytic cells following Pam3CSK4 or LPS exposure with or without candesartan as detailed in Section 2. Blot shown here is a representative of n = 4 experiments.