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. Author manuscript; available in PMC: 2009 Oct 30.
Published in final edited form as: Nature. 2009 Mar 8;458(7242):1201–1204. doi: 10.1038/nature07844

Figure 2. Reconstitution of the PBSPro binding complex with recombinant proteins.

Figure 2

(A) 50 ng or 250 ng of recombinant GST-tagged ZFP124, L1td1 and ZFP809 added to EMSA reactions with radiolabeled labeled probes corresponding to PBSPro (PRO) or PBSB2 (B2). (B) EMSAs performed as above, with PCC4 EC nuclear extract or 25, 50, or 100 ng of GST-ZFP809 protein and 100 ng, 200 ng or 500 ng of GST-TRIM28 of protein as indicated.