Figure 4. Expression of ZFP809 in a differentiated cell line causes a potent block to the replication of PBS^Pro utilizing retroviruses.

(A) 293A, control Clone 1, Clone 9 and Clone 5 cell lines were infected with either PBS^Pro or PBS^B2 VSV-G pseudotyped MLV virions expressing the puromycin resistance gene. Infection efficiency in each cell line was monitored by colony count after of puromycin selection. Graph shows ratio of B2/PRO infection efficiency in each cell line, normalized with 293A =1. Error bars show +/− standard error, with n=3. (B) Control Clone 1, Clone 9 and Clone 5 cell lines were infected at a low multiplicity with replication-competent amphotropic MLV utilizing either a PBS^Pro (PRO) or PBS^Q (Q). Viral spread in these cell lines was monitored by analysis or reverse transcriptase activity²² in the culture media every day for 6 days. (C) HTLV-1 LTR or HIV-1 LTR firefly luciferase reporter activity monitored in cell lines control Clone 1, Clone 5, and Clone 9 which were simultaneously transfected with increasing amounts of a Tax expressing vector (pTAX) or TAT expressing vector (pTAT-HA) as shown. Firefly luciferase values normalized to renilla luciferase values from a simultaneously transfected renilla control vector. Error bars show +/− standard error, with n=3.