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Morphologic localization of YN- and YC-tagged secretin receptor constructs. Shown are representative confocal immunofluorescence images illustrating cell surface localization of HA-tagged secretin receptors expressed on the surface of non-permeabilized COS cells, as indicated. Similar to wild type secretin receptor, the receptor constructs tagged with non-fluorescent YFP fragments were able to traffic normally to the plasma membrane. Bar, 25 μm.
