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. Author manuscript; available in PMC: 2009 Nov 1.
Published in final edited form as: Biochim Biophys Acta. 2008 Jul 17;1778(11):2555–2563. doi: 10.1016/j.bbamem.2008.07.008

Fig 4.

Fig 4

Fluorescence spectra and anisotropy of receptor tagged with fragments of YFP. Shown are fluorescence emission spectra (left panel) and steady state fluorescence anisotropy data (right panel) for intact YFP-tagged secretin receptor and YFP reconstituted from its fragments attached to secretin receptor constructs that were co-expressed in COS cells. The cells were excited at 480 nm and emission was acquired from the region between 500 nm and 600 nm. Data represent means ± S.E.M. of values from four independent experiments performed in duplicate.