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Immunolabeling for glutamic acid decarboxylase (GAD) and ecto-nucleoside triphosphate diphosphohydrolase 3 (NTPDase3) immunoreactivities in the hypothalamus using the mirror technique. Corresponding arrows point to matching cell halves immunostained either for GAD (A) or NTPDase3 (B). None of the examined GAD-immunoreactive neurons contained NTPDase3-immunoreactive material. Asterisks label corresponding vessels. Scale: 50 μm.
