Table 1.
Primer name | Primer sequence 5′ to 3′ |
---|---|
Gimap8-f | CCAGGAGACCCAGGTGAAAG |
Gimap8-r | AGTTGAATGCTCATCATAGCTCCTT |
Gimap8-p | 6FAM-TCTGTTGACAATAGCCAATGATCTCA-BHQ1 |
| |
Gimap9-f | AGGAACGGCAGAGCCTACTTT |
Gimap9-r | CCACTAGACATTGGTTCAGCTTCTTA |
Gimap9-p | 6FAM-CTGACAGGATATATAAGGACA-MGBQ |
| |
Gimap4-f | AACATGCCGTACAGAGCTCACA |
Gimap4-r | AGTGGCACCATTAGAAGGCAAA |
Gimap4-p | 6FAM-CCATGACACACCCACTCCAACAGGG-BHQ1 |
| |
Gimap6-f | TGGATGCTCTGGATGTTGCA |
Gimap6-r | TCCTGCTCATCCCCTTGTG |
Gimap6-p | 6FAM-TTGTTGAAGCCACAATGGCGTCTCTCA-BHQ1 |
| |
Gimap7-f | GGACTCAGTGTCAGGCTCCAA |
Gimap7-r | CGGGAGGACAGGCTAGCATA |
Gimap7-p | 6FAM-CTGGATCACACTTGGCGCTCAGCTC-BHQ1 |
| |
Gimap1-f | AGAGGCGGACCAGGTTCCTA |
Gimap1-r | CCTCCAGCCCTGCCTGTAG |
Gimap1-p | 6FAM-TTCTGCCATCTCCACAGCCCA-BHQ1 |
| |
Gimap5-f | CATGTTAGGGAAGCTCAGTC |
Gimap5-r | GAAGGGTTCTACTGTGTCTCA |
Gimap5-p | 6FAM-TTTCACTATCATTTGACTCCTGTGCA-BHQ1 |
| |
Gimap3-f | CCACAGGGAGTGTAGACCTTGAA |
Gimap3-r | CTGCTGTTTCCGAATCCAGTTT |
Gimap3-p | 6FAM-ATCCTCCAGCGTCCAC-MGBQ |
| |
Lr8-f | GCCTCTGGTTGTGCCTTCTG |
Lr8-r | CCCTGTCCCATCTCATGGAT |
Lr8-p | 6FAM-CCCACTCCAGCCAAAATTGCCACA-BHQ1 |
| |
Cyc-f | CACCGTGTTCTTCGACAT |
Cyc-r | TTTCTGCTGTCTTTGGAACT |
Cyc-p | HEX-CTGCTTCGAGCTGTTTGCAGAC-BHQ1 |
Probes and primers were designed to bind near the 3′ end of the transcripts. f is forward primer, r is reverse primer, p is probe, 6FAM is 6-carboxyfluorescein, HEX is hexachlorofluorescein, and BHQ1 is black hole quencher 1.