Table 1.
Plasmids used in this study.
| Plasmid | Description | Source |
|---|---|---|
| GFP-SDEL | ER-retained GFP-SDEL in pENTR2/3 | van Dooren et al. (2005) |
| PfGRASP-GFP | PfGRASP-GFP in pARL1a- | Struck et al. (2005) |
| pETGEXCT | GST-tag bacterial expression vector | Sharrocks (1994) |
| pTY40 | GST fusion of yeast Sar1 in pGEX-2T bacterial expression vector | Barlowe et al. (1994) |
| pETGEX-PfSar1 | PfSar1 in pETGEXCT bacterial expression vector | This study |
| pPM-campro-CRT-Flag-GFP | PfCRT-GFP, 1 kb calmodulin 5′ UTR (human dhfr) | This study |
| pML-campro-GFP(N-C) | GFP-GFP tandem, 1 kb calmodulin 5′ UTR (human dhfr) | This study |
| pML1 | GFP-PfSec12, 1367 bp pfsec12 5′ UTR (human dhfr) | This study |
| pML2 | mRFP-PfSec12, 1367 bp pfsec12 5′ UTR (bsd) | This study |
| pML3 | mRFP-PfSec12, 1 kb calmodulin 5′ UTR (bsd) | This study |
| pML4 | PfSec24a-GFP, 1230 bp pfsyn17 5′ UTR (human dhfr), Rep20 | This study |
| pML5 | PfSec24a-mRFP, 1230 bp pfsyn17 5′ UTR (bsd) | This study |
| pML6 | yeast GFP-Sys1 (WT), 1367 bp pfsec12 5′ UTR (human dhfr) | This study |
| pML7 | yeast GFP-Sys1 (AxA), 1367 bp pfsec12 5′ UTR (human dhfr) | This study |