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. Author manuscript; available in PMC: 2010 Feb 1.
Published in final edited form as: Biochim Biophys Acta. 2008 Dec 16;1791(2):92–102. doi: 10.1016/j.bbalip.2008.12.002

Figure 10. Effect of cPLA2 and sPLA2 inhibitors on β-glucuronidase release from A23187-stimulated human lung macrophages.

Figure 10

Human lung macrophages were preincubated (30 min, 37°C) with increasing concentrations (0.01–10 μM) of pyrrolidine-1, AZ-1 or Me-Indoxam and then stimulated with 1 μM A23187 (90 min, 37°C). At the of the incubation, supernatants were collected and centrifuged twice (1000 g, 4°C, 5 min). β-Glucuronidase release was determined by a colorimetric technique. The values are expressed as the percentage of the total cellular content determined in cell aliquots lysed with 0.1% Triton X-100. The data are the mean ± SE of three different experiments. § p < 0.01 vs. unstimulated.