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. Author manuscript; available in PMC: 2010 Feb 1.
Published in final edited form as: Biochim Biophys Acta. 2008 Dec 16;1791(2):92–102. doi: 10.1016/j.bbalip.2008.12.002

Figure 5. Effect of cPLA2 and sPLA2 inhibitors on AA release from PMA + A23187-stimulated human lung macrophages.

Figure 5

[3H]AA-labeled human lung macrophages were preincubated (30 min, 37°C) with increasing concentrations (0.01–10 μM) of AZ-1 (○), pyrrolidine-1 (●) or Me-Indoxam (■) and then stimulated with 1 μM PMA (10 min, 37°C) and subsequently with 1 μM A23187 (30 min, 37°C). At the of the incubation, supernatants were collected and centrifuged twice (1000 g, 4°C, 5 min) for subsequent determination of AA release. Values are the mean ± SE of three different experiments.

* p < 0.05 vs. PMA + A23187

** p < 0.01 vs. PMA + A23187