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. Author manuscript; available in PMC: 2010 Feb 1.
Published in final edited form as: Biochim Biophys Acta. 2008 Dec 16;1791(2):92–102. doi: 10.1016/j.bbalip.2008.12.002

Figure 9. Effect of cPLA2 inhibitors on PAF synthesis from A23187-stimulated human lung macrophages.

Figure 9

Human lung macrophages were preincubated (30 min, 37°C) with increasing concentrations (0.01–10 μM) of AZ–1 (○) or pyrrolidine–1 (●) and then stimulated with 1 μM A23187 (10 min, 37°C) in the presence of [3H]acetic acid. At the end of the incubation, lipid were extracted from both cells and supernatants by the Bligh and Dyer technique. PAF was separated by TLC and quantitated by lipid scintillation counting. Values are the mean ± SE of three different experiments.

** p < 0.01 vs. A23187 alone