Fig. 7.

Real-time PCR analyses for mRNA expressions of bone-related genes in different cells after cultured for (a) 3 d and (b) 14 d

For Runx2, collagen I (colI) and bone sialoprotein (BSP) (Days 3 and 14) and osteocalcin (OCN) (Day 14), the increased expression on pCTGF-MSCs or MSCs cultured in OS-medium was observed. However, mock-MSCs and normal control MSCs only have basal expressions of these osteoblastic phenotype genes. GAPDH was used as internal control. ^* P<0.05, statistically significant difference between control cells and pCTGF-MSCs or MSCs cultured in OS-medium