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. Author manuscript; available in PMC: 2010 Mar 1.
Published in final edited form as: Mol Immunol. 2009 Feb 1;46(6):1020–1030. doi: 10.1016/j.molimm.2008.08.271

Fig. 1.

Fig. 1

Binding properties of chimeric CD134L fusion proteins. (A) The amino acid sequences of feline and human CD134L were compared and regions of homology (dark shading) identified. Start of the TNF homology domain (THD) and chimera junction are arrowed. Residues predicted to lie at the CD134 binding face of CD134L are in blue boxes while aa F180, critical for the CD134:CD134L interaction(Compaan and Hymowitz, 2006) is circled. (B) Predicted 3D-structure of the feline CD134L (blue) and CD134 (gray) complex illustrating residues at the binding face of CD134L (dark blue) and residue F180(Compaan and Hymowitz, 2006) (arrowed). (C) Binding of FLAG-tagged fCD134L, hCD134L, mCD134L and the human/feline and feline/human chimeric CD134Ls (h/fCD134L and f/hCD134L) to MCC cells expressing feline or human CD134. Bound ligands were detected with anti-FLAG followed by PE-anti-mouse IgG. Cells were processed for flow cytometry and histograms represent 10,000 events collected in LIST mode, bars illustrate percentage positive. Histograms are representative of two independent analyses.